Translation Initiation: Extract Systems and Molecular Genetics

Translation Initiation: Extract Systems and Molecular Genetics

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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.5.4. Immunoprecipitation reaction and detection of precipitated proteins 1. Add 200 mg WCE (as estimated by the Bradford assay) to ... portion (usually 10 ml or 5%) by immunoblotting. 4. Wash the resin three times with 200 ml of Buffer A containing 0.1% of Triton X-100. ... problem with this assay occurs when the epitope is sterically sequestered. ... REFERENCES Asano, K., and Hinnebusch, A. G. (2001).


Title:Translation Initiation: Extract Systems and Molecular Genetics
Author:
Publisher:Academic Press - 2007-10-15
ISBN-13:

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